About MetaMap

A suite of metaproteomics data visualization tools.

This project was created in the summer of 2019 and is supported by The Figeys Lab at the uOttawa Faculty of Medicine. We plan to integrate this app as the data visualization module for iMetaLab 2.0. Features and bug fixes are being pushed out every week.

Thomas Hai Li
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Browsing Different Samples

All of your samples have been loaded in the list below. Clicking on a selection will display their corresponding MS intensities.

Changing Files

You may upload another file if you'd like. They should be of type csv and be in the correct format. This sample file demonstrates the proper formatting; there are 3 columns. The column headers are in bold below...

File Formatting
  1. id: The complete taxonomic path/identity, delimited by "@".
  2. value: The average MS intensity across all samples.
  3. *sample intensities* : The MS intensity of each sample, delimited by ";". The header title should list the name of all samples, also delimited by ";". There must be no spacing in the header title.
Example
id value s1;s2;s3
cellular organisms@Bacteria 580970.92 1039022.58;703890.17;0
Circle Packing
Chart Description

Circle packing gives a broad overview of relative proportions between MS-identified taxa.

Interaction

Click on a taxon node to travel towards that taxon. The subtaxa names for that node will be displayed.

Toolbar Options

Increase or decrease the font size of taxon labels

Context Menu Options (right-clicking on a node)

Compare MS-intensities of a taxon accross all samples

Compare MS-intensities of subtaxa within this sample

Treemap
Chart Description

Treemaps use nested rectangles to display hierarchical data. Combined with a dynamic color scheme, this visualization can create what can be labelled as abstract art :P Each rectangle represents a single taxon at the given depth and its area is proportional to its MS intensity. It must be noted that for every node, unknown peptides that contribute to the intensity have been excluded to draw the treemap without gaps. To include unknown peptide intensities, select the toggle button in the toolbar. However, this may result in gaps between the rectangles.

Interaction

Zoom in and out with the scroll wheel and pan the view by clicking + dragging.

Toolbar Options

Change the current taxonomic level / draw depth

Increase or decrease the font size of taxon labels

Include or exclude unknown peptides

Context Menu Options (right-clicking on a rectangle)

Compare MS-intensities of a taxon accross all samples

Compare MS-intensities of subtaxa within this sample

Sunburst
Chart Description

Sunbursts clearly display taxa proportions. That's it.

Interaction

Click on an arc visit that taxon. The corresponding proportion for that node will be displayed.

Toolbar Options

None at the moment

Context Menu Options (right-clicking on an arc)

None at the moment

Horizontal and Radial Tree
Chart Description

Trees are great for visualizing diversity and relationships between taxa. In the horizontal tree, nodes are sorted by their MS intensity in descending order (greatest value nodes at the top of the chart). The radial tree intuitively shows the proportion of taxa with their circular nature and with the help of a color scheme, mimicking a pie chart.

Interaction
  • Zoom in and out with the scroll wheel and pan the view by right-clicking + dragging.
  • Collapse a node by left-clicking on it. This may also yield big performance boosts if a lot of irrelevant nodes are collapsed.
  • Select multiple nodes with ctrl+left-click. For now, this function is only useful for coloring multiple nodes at a time but will be expanded on in the future (ie. to carry further analysis).
Toolbar Options

Zoom in or out

Toggle and increase/decrease font-size of taxon labels

Normalize radius of all nodes

Customize color palette and theme

The slider colors nodes based on different taxonomic levels

Context Menu Options (right-clicking on a node)

Compare MS-intensities of this taxon accross all samples, or of subtaxa within this sample

Collapse all other nodes, or expand child nodes here

Select this node, direct subnodes, or all subnodes

Color selected nodes

Hierarchical Bar Chart
Chart Description

This chart displays quantitative information with the least amount of distraction.

Interaction

Jump down a taxonomic rank by clicking on a bar, or jump up by clicking on the background.

Toolbar Options

None at the moment

Context Menu Options (right-clicking on a bar)

Compare MS-intensities of a taxon accross all samples

Compare MS-intensities of subtaxa within this sample

Oh Snap

The file did not match the correct format and was not loaded.